DEAE-Dextran

CAS Number: 9064-91-9

DEAE-Dextran (Di-ethyl-amino-ethyl Dextran) is a polycationic derivative of Dextran. It is a white, hygroscopic powder, freely soluble in water and salt solutions which can be sterilized by autoclaving or by sterile filtration.

DEAE-Dextran maintains its quality and efficacy for more than 3 years when stored at room temperature in airtight packaging. The product is supplied as the hydrochloride.

Pack sizes

Item number Weight Packaging
17-0350-01 100 g 180 mL polypropylene (PP) bottle
17-0350-02 500 g 880 mL low-density polyethylene (LDPE) bottle
17-0350-03 5 kg 8.4 L polypropylene (PP) bucket. Powder sealed in double Low-density polyethylene (LDPE) bags
17-0350-07 40 kg 60 L steel drum. Powder sealed in double low-density polyethylene (LDPE) bags
17-0350-60 Bulk Steel drum. Powder sealed in double low-density polyethylene (LDPE) bags

Applications

DEAE-Dextran offers a wide range of chemical and biological properties. The applications below are well documented.


Adjuvant in vaccines

DEAE-dextran is a well-known adjuvant in vaccines.1 The DEAE-dextran will help create a strong immune reaction in the human or animal receiving the vaccine.


Agent for transfection

DEAE-dextran can be used to transfer nucleic acid into cells. Due to the cationic properties of DEAE-dextran, it will associate tightly with the anionic backbone of the nucleic acid 2,3. This will create a net-positive complex which can adhere to the cell membrane and enter the cytoplasm via endocytosis or an induced osmotic shock.

Stabilizer of proteins

DEAE-dextran has shown to stabilize both lyophilized proteins and enzyme as well as proteins in solutions4.


Agent for reduction of cholesterol count

Clinical studies of DEAE-dextran have shown that it can reduce both blood cholesterol and triglycerides.4


Enhancer of viral infectivity

Studies have shown that DEAE-dextran can increase the infectivity of viruses.5

 

References

  1. Coffman, R. L., Sher, A. & Seder, R. A. Vaccine Adjuvants: Putting Innate Immunity to Work. Immunity 33, 492–503 (2010).
  2. Lalani, J. & Misra, A. 4 - Gene Delivery Using Chemical Methods. in Challenges in Delivery of Therapeutic Genomics and Proteomics (ed. Misra, A.) 127–206 (Elsevier, 2011). doi:10.1016/B978-0-12-384964-9.00004-9.
  3. Spain, S. G. et al. 4.424 - Nanoparticles for Nucleic Acid Delivery. in Comprehensive Biomaterials (ed. Ducheyne, P.) 389–410 (Elsevier, 2011). doi:10.1016/B978-0-08-055294-1.00133-1.
  4. de Belder, A. N. Dextran Handbook. (Amersham Biosciences, 2003).
  5. Pagano, J. S., McCutchan, J. H. & Vaheri, A. Factors Influencing the Enhancement of the Infectivity of Poliovirus Ribonucleic Acid by Diethylaminoethyl-Dextran. J. Virol. 1, 891–897 (1967).

Specification

Test/Characteristic Limit Test Method
Appearance Visual inspection EP
Identification Complies EP
Nitrogen content, %N 2.8-3.3 EP
Specific optical rotation +140 to +150 EP
Specific viscosity 0.5-1.0 EP
Absorbance, (375 nm) Max 0.1 EP and USP
Heavy metals; ug/g ≤ 5 USP
Loss on drying, % ≤ 7 EP and USP
pH, (1 % w/v sol) 4.0-6.0 EP
Residue on ignition; % ≤ 1.0 USP
Microbiological Contamination:
TYMC, cfu/g ≤ 100 EP / USP
TAMC, cfu/g ≤ 100 EP / USP

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